我们研究小组的初步研究结果显示,蔬果废物含有丰富的可溶性糖类,含水量高,两者合而为一,提供适宜的培养基,可令蔬果生物量迅速退化。 本课题促使我们寻找一种适合作为动物饲料来源的FVD生物质高效储存和安全利用的防腐剂。 由于价格低廉、易取、操作安全,偏亚硫酸钠(SMB)是我们的首选材料。 我们研究小组最近进行的实验室和大规模实验显示,以每公斤6克湿生物质的速度施用的中小型企业,对各种微生物提供强力的抑制作用,从而在好氧和厌氧贮存下有效地保存FVD。

由于FVD含水量高,运输和处理面临挑战。 将它们与干饲料混合并封存起来是最简单和最有效的方法之一。
i) 避免与烘干有关的高昂能源成本;
ii)减少所产生的污水量;及
iii)协助提高用作动物饲料的FVD的营养价值和保存稳定性。
总混合口粮生产商越来越有兴趣为反刍动物生产系统制造可运输袋式筒仓(容量为300至400公斤)的TMR,这样可以有效地厌氧发酵,储存过程中营养损失最小。 通过这种方法,可以降低人工费,增加容易腐烂的高水分副产品,在青贮过程中也会发生一些营养素变化,所有这些都提高了作为反刍动物食物的TMR青贮的价值。 乳酸发酵TMR成分有助于掩蔽FVD等无吸引力成分的风味,具有重要意义。 这种方法通过在青贮期间发酵,将FVD添加到TMR中,从而最大限度地减少饲料外的问题,并改善它们的气味和风味。

随着发酵的进行,青贮微生物群落中乳酸菌(LAB)的快速高效发展是生产优质青贮饲料的关键因素之一[8]。 与其他微生物比较,我们的研究显示LAB是最容易受中小型企业杀菌影响的微生物。最近的研究显示,以每公斤新鲜生物量5毫升的速度,加入苯甲酸钠(200g/L)、山梨酸钾(100g/L)和亚硝酸钠(50g/L)的水溶液,可提高微生物的存活率。不同作物的好氧稳定性与抑制不良菌群的生长有很大关系。 以苯甲酸钠、山梨酸钾和亚硝酸钠为原料,采用相同的化学混合物(CM)经Knicky和Spöndly以5mL/kg的新鲜作物作原料。

据这些作者说,青贮发酵过程以LAB为主,他们指出,与对照组相比,CM处理在过程结束时导致pH值显著降低,乳酸浓度显著升高。 为了减少SMB对LAB生长和生存的负面影响,进行了两个系列的实验。 第一种策略涉及探讨是否可能分离出一种对中小型企业具有耐药性的乳酸菌株,理论上,这种菌株可以在含有中小型企业青贮生物量的厌氧条件下繁衍。 第二种策略是用不抑制LAB生长发育的CM代替部分SMB,以减轻SMB的生长发育。我们预测,减少LAB的生长发育,可使LAB在青贮料中生长发育。

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